Abstract

Short Review

High-Performance Liquid Chromatography (HPLC): A review

Abdu Hussen Ali*

Published: 20 June, 2022 | Volume 6 - Issue 1 | Pages: 010-020

Today HPLC is widely applied for separations and purifications in a variety of areas including pharmaceuticals, biotechnology, environmental, polymer and food industries. It is accomplished by injection of a small amount of liquid sample into a moving stream of liquid (called the mobile phase) that passes through a column packed with particles of the stationary phase. The separation of a mixture into its components depends on different degrees of retention of each component in the column. HPLC is just one type of liquid chromatography, meaning the mobile phase is a liquid. Reversed-phase HPLC is the most common type of HPLC. The reversed-phase means the mobile phase is relatively polar, and the stationary phase is relatively non-polar. HPLC instrumentation includes a Solvent reservoir, pump, injector, column, detector, and integrator or acquisition and display system. The heart of the system is the column where separation occurs. The information that can be obtained using HPLC includes identification, quantification, and resolution of a compound. The major applications are in the area of Pharmaceuticals, food, research, manufacturing, forensics, and bio-monitoring of pollutants.

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Keywords:

Column; Mobile phase; Separation; Stationary phase

References

  1. Gerber F, Krummen M, Potgeter H, Roth A, Siffrin C, Spoendlin C. Practical aspects of fast reversed-phase high-performance liquid chromatography using 3 microm particle packed columns and monolithic columns in pharmaceutical development and production working under current good manufacturing practice. J Chromatogr A. 2004 May 21;1036(2):127-33. doi: 10.1016/j.chroma.2004.02.056. PMID: 15146913.
  2. Xiang Y, Liu Y, Lee ML. Ultrahigh pressure liquid chromatography using elevated temperature. J Chromatogr A. 2006 Feb 3;1104(1-2):198-202. doi: 10.1016/j.chroma.2005.11.118. PMID: 16376355.
  3. Bergh JJ, Breytenbach JC. Stability-indicating high-performance liquid chromatographic analysis of trimethoprim in pharmaceuticals. J Chromatogr. 1987 Jan 30;387:528-31. doi: 10.1016/s0021-9673(01)94565-0. PMID: 3558640.
  4. Haginaka J, Yasuda H, Uno T, Nkagawa T. Alkaline degradation and determination by high-performance by high-performance liquid chromatography. Chemical Pharmacy. Bullet. 1984; 32: 2752-2758
  5. Fredj G, Paillet M, Aussel F, Brouard A, Barreteau H, Divine C, Micaud M. Determination of sulbactam in biological fluids by high-performance liquid chromatography. J Chromatogr. 1986 Nov 28;383(1):218-22. doi: 10.1016/s0378-4347(00)83464-7. PMID: 3029153.
  6. Ayrton J. Assay of ceftazidime in biological fluids using high-pressure liquid chromatography. J Antimicrob Chemother. 1981 Sep;8 Suppl B:227-31. doi: 10.1093/jac/8.suppl_b.227. PMID: 19802990.
  7. Polite L. Liquid chromatography: basic overview. In: Miller J, Crowther JB [eds], Analytical chemistry in a GMP environment: a practical guide. John wiley & sons, New York.
  8. United States Pharmacopoeia. 27th ed. The USP Convention Inc., Rockville, MD.
  9. Rodenas V, Garcia MS, Sanchez-Pedreno C, Albero MI. Flow-injection spectrophotometric determination of frusemide or sulphathiazole in pharmaceuticals. Journal of Pharmacy and Biomedical Analyst. 1997; 15: 1687-1693.
  10. Chemical Analysis Modern Instrumentation Methods and Techniques, Second Edition, Francis Rouessac and Annick Rouessac, University of Le Mans, France.
  11. Abidi SL. High-performance liquid chromatography of phosphatidic acids and related polar lipids. Journal of Chromatography. 1991; 587: 193-203.
  12. The European Pharmacopoeia. fourth ed., Council of Europe, Strasbourg.
  13. Tsai IL, Weng TI, Tseng YJ, Tan HK, Sun HJ, Kuo CH. Screening and confirmation of 62 drugs of abuse and metabolites in urine by ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. J Anal Toxicol. 2013 Nov-Dec;37(9):642-51. doi: 10.1093/jat/bkt083. Epub 2013 Sep 30. PMID: 24084874.
  14. Hearn MTW. Ion-pair chromatography on normal and reversed-phase systems. Advance Chromatography. 1980; 18: 59–100.
  15. Siddiqui MR, AlOthman ZA, Rahman N. Analytical techniques in pharmaceutical analysis: A review. Arabian Journal of Chemistry.

Willard H, Merritt L, Dean J, Settle F. Instrumental Methods of Analysis, 7th edn, Wadsworth Publishers, Stamford, CT. 1998.

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