Abstract

Research Article

Synthesis of citric acid using novel Aspergillus niveus obtained from agricultural wastes

R Gnanasekaran#, A Petchiammal, BD Subhashree, M Anubha and Yuvaraj Dinakarkumar*

Published: 13 September, 2022 | Volume 6 - Issue 1 | Pages: 051-055

Fungus belonging to the genus Aspergillus is considered highly important in the production of various types of enzymes and organic acids. Aspergillus species produce organic acids such as citric acid, itaconic acid, and malic acid, which are one of the most important alternate techniques for chemical processes. Citric acid is an important component in the manufacturing process of food and beverages, pharmaceuticals, cosmetics, toiletries, detergents, and other industries. In this work, A.niveus was isolated from the agricultural waste collected in Kotagiri, The Nilgiris, India. Submerged batch fermentation with a range of low-cost substrates, such as wheat flour, corn starch, and sweet potato, was used to successfully synthesize citric acid by the isolated fungus. In addition, production-related factors such as substrate concentration and incubation time were optimized. The maximum yield of citric acid was produced using A. niveus from corn starch at a concentration 7of 120 g/L after 168 hours at pH 3.2. Furthermore, with a degree of extraction of 91.96, citric acid was extracted from fermentation.

Read Full Article HTML DOI: 10.29328/journal.aac.1001032 Cite this Article Read Full Article PDF

Keywords:

Aspergillus niveus; Citric acid; Extraction; Fermentation; Organic acid

References

  1. Yang L, Lübeck M, Lübeck PS. Aspergillus as a versatile cell factory for organic acid production. Fungal Biology Reviews. 2017; 31(1):33-49.
  2. Bennett JW. An overview of the genus Aspergillus. Aspergillus: molecular biology and genomics. 2010; 1-7.
  3. Sun X, Wu H, Zhao G, Li Z, Wu X, Liu H, Zheng Z. Morphological regulation of Aspergillus niger to improve citric acid production by chsC gene silencing. Bioprocess Biosyst Eng. 2018 Jul;41(7):1029-1038. doi: 10.1007/s00449-018-1932-1. Epub 2018 Apr 2. PMID: 29610994.
  4. Alekseev VK, Dubina MV, Komov VP. Molecular-genetic and biochemical characteristics of citrate synthase from the citric-acid producing fungus Aspergillus niger. Applied Biochemistry and Microbiology. 2016; 52(9):810-817.
  5. Kubicek CP, Röhr M, Rehm HJ. Citric acid fermentation. Critical Reviews in Biotechnology. 1985; 3(4):331-73.
  6. Li G, He D, Qian Y, Guan B, Gao S, Cui Y, Yokoyama K, Wang L. Fungus-mediated green synthesis of silver nanoparticles using Aspergillus terreus. Int J Mol Sci. 2012;13(1):466-76. doi: 10.3390/ijms13010466. Epub 2011 Dec 29. PMID: 22312264; PMCID: PMC3269698.
  7. Kumar D, Jain VK, Shanker G, Srivastava A. Citric acid production by solid-state fermentation using sugarcane bagasse. Process Biochemistry. 2003; 38(12):1731-1738.
  8. Vandenberghe LP, Soccol CR, Pandey A, Lebeault JM. Solid-state fermentation for the synthesis of citric acid by Aspergillus niger. Bioresource Technology. 2000; 74(2):175-8.
  9. Shishikura A, Takahashi H, Hirohama S, Arai K. Citric acid purification process using compressed carbon dioxides. The Journal of Supercritical Fluids. 1992; 5(4):303-312.
  10. Papagianni M. Advances in citric acid fermentation by Aspergillus niger: biochemical aspects, membrane transport and modeling. Biotechnol Adv. 2007 May-Jun;25(3):244-63. doi: 10.1016/j.biotechadv.2007.01.002. Epub 2007 Jan 19. PMID: 17337335.
  11. Max B, Salgado JM, Rodríguez N, Cortés S, Converti A, Domínguez JM. Biotechnological production of citric acid. Braz J Microbiol. 2010 Oct;41(4):862-75. doi: 10.1590/S1517-83822010000400005. Epub 2010 Dec 1. PMID: 24031566; PMCID: PMC3769771.
  12. Chergui D, Akretche-Kelfat S, Lamoudi L, Al-Rshaidat M, Boudjelal F, Ait-Amar H. Optimization of citric acid production by Aspergillus nigerusing two downgraded Algerian date varieties. Saudi J Biol Sci. 2021 Dec;28(12):7134-7141. doi: 10.1016/j.sjbs.2021.08.013. Epub 2021 Aug 9. PMID: 34867016; PMCID: PMC8626340.
  13. Kulprathipanja S. Inventor; UOP LLC, assignee. Separation of citric acid from fermentation broth with a neutral polymeric adsorbent. United States patent US 4,720,579. 1988.
  14. Wu J, Peng Q, Arlt W, Minceva M. Model-based design of a pilot-scale simulated moving bed for purification of citric acid from fermentation broth. J Chromatogr A. 2009 Dec 11;1216(50):8793-805. doi: 10.1016/j.chroma.2009.03.028. Epub 2009 Mar 17. PMID: 19344909.
  15. Zhu S, Zhao X, Song Y, Lu S, Yang B. Beyond bottom-up carbon nanodots: Citric-acid derived organic molecules. Nano Today. 2016. 11(2):128-132.

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