Published: 29 January, 2025 | Volume 9 - Issue 1 | Pages: 001-006
Figure 3:
(A) - Chromatogram showing the elution profile of the recombinant nanobody purification by HIC using Seplife® Phenyl Large Scale HP. The elution fractions from the previous IEC step have been conductivity and pH adjusted and loaded on the column at 5 minutes retention time. A wash step at 0.35 M ammonium sulphate in PB and elution step with 0.2 M ammonium sulphate in PB pH 7.0 were applied. The elution fraction of 23 ml was collected representing the final product. (B) Reduced SDS-PAGE indicating the purity of different elution fractions.
Read Full Article HTML DOI: 10.29328/journal.aac.1001053 Cite this Article Read Full Article PDF
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