Figure 3

Efficient Sequential Chromatographic Purification of a Recombinant Nanobody-Fc Fusion Designed for Treatment of Severe Fever with Thrombocytopenia Syndrome

Simona Serban*, Long Liu, Yan Liu, Xiaoju Lei, Cheng Zhang, Yanjun Li, Xiaokang Kou and Alessandra Basso

Published: 29 January, 2025 | Volume 9 - Issue 1 | Pages: 001-006

aac-aid1053-g003

Figure 3:

(A) - Chromatogram showing the elution profile of the recombinant nanobody purification by HIC using Seplife® Phenyl Large Scale HP. The elution fractions from the previous IEC step have been conductivity and pH adjusted and loaded on the column at 5 minutes retention time. A wash step at 0.35 M ammonium sulphate in PB and elution step with 0.2 M ammonium sulphate in PB pH 7.0 were applied. The elution fraction of 23 ml was collected representing the final product. (B) Reduced SDS-PAGE indicating the purity of different elution fractions. 

Read Full Article HTML DOI: 10.29328/journal.aac.1001053 Cite this Article Read Full Article PDF

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