A validated method for coumarin quantification in Meliloti herba and its ethanolic extracts using micellar thin-layer chromatography

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Submitted: September 24, 2021
Published: Oct 12, 2021
DOI: 10.29328/journal.aac.1001024
Keywords:
Coumarin, Micellar thin-layer chromatography, Meliloti herba, Validation

Main Article Content

Aleksey G Verushkin
Laboratory of Pharmacopoeial Analysis, Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines, 33 Astronomicheskaya str, Kharkiv, 61085, Ukraine
Artem Yu Kulikov
Laboratory of Pharmacopoeial Analysis, Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines, 33 Astronomicheskaya str, Kharkiv, 61085, Ukraine
Andranik A Kutsanyan
Department of Chemistry of Natural Compounds and Nutriciology, National University of Pharmacy, 53 Pushkinska str, Kharkiv, 61022, Ukraine

Abstract

A micellar thin-layer chromatography method for the quantitative determination and validation of coumarin in Meliloti herba and its ethanolic extracts was developed and validated. For achieving good determination, the mobile phase of 5x10-4 mol/L Tween-80 in a mixture propanol-2 – water (5:95 v/v) was used. Densitometric determination was carried out at 275 nm. The calibration curve was linear in the range of 0.1-2.5 μg per band. The proposed method is simple, rapid, precise and accurate; replacing hazardous solvents by greener ones correspond to the modern requirements in “Green chemistry” concepts. The obtained data can be used for the routine analysis of coumarin in medical plant and extracts.

Article Details

Verushkin, A. G., Kulikov, A. Y., & Kutsanyan, A. A. (2021). A validated method for coumarin quantification in Meliloti herba and its ethanolic extracts using micellar thin-layer chromatography. Annals of Advances in Chemistry, 5(1), 013–018. https://doi.org/10.29328/journal.aac.1001024
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Copyright (c) 2021 Verushkin AG, et al.

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